Table 2 iTRAQ quantitative mass spectrometry analysis using OMPs from each ON/OFF pair.

From: A biphasic epigenetic switch controls immunoevasion, virulence and niche adaptation in non-typeable Haemophilus influenzae

Gene ID

Gene

Ratio ON:OFF*

Stouffers P-value

Stouffer Adj

Increased expression in 723 modA2 ON

 723_01555

N-acetylneuraminate epimerase NanM

2.36

5.40E−14

6.32E−13

 723_01788

Adhesin translocation protein HMW2B

2.05

0

0

Decreased expression in 723 modA2 ON

 723_01435

Haem/haemopexin utilization protein C HxuC1

0.28

0

0

 723_00620

Transferrin-binding protein 1

0.32

4.74E−08

3.45E−07

 723_01615

Major ferric iron-binding protein HitA†

0.37

0

0

 723_01434

Haem/haemopexin transporter protein HxuB†

0.41

0

0

 723_01596

Haemin receptor, HemR

0.42

0

0

 723_01306

15 kDa peptidoglycan-associated lipoprotein OMP P6

0.6

2.57E−04

1.12E−03

Increased expression in C486 modA4 ON

 C486_00892

OMP P2

1.52

0

0

Increased expression in 477 modA5 ON

 477_00572

OMP P5

1.65

0

0

Increased expression in R2866 modA10 ON

 R2866_0725

Adhesin Hia‡§

11.53

0

0

 R2866_0192

OMP P6

1.85

0.046

0.17

 R2866_1237

OMP P5

1.65

7.39E−03

0.043

  1. Gene designations from analysis with our SMRT produced genomes for each strain; differences are represented as ON:OFF; statistical significance is measured using Stouffers P value and Stouffers adjusted (Adj) value, as the results are the interpretation and comparison of two independently prepared sets of OMPs from each ON/OFF pair. Schematics of differentially regulated genes with locations of ModA methylation motifs are depicted in Supplementary Fig. 3.
  2. *Only samples with an ON:OFF ratio >1.5 or <0.7 were included. Complete iTRAQ data for all the five strain pairs are presented as Supplementary Data 2.
  3. †Indicates identification by microarray and iTRAQ.
  4. ‡Identified by western blotting and iTRAQ.
  5. §Hia was subsequently shown to be regulated in a modA10-independent manner.
Back to article page