Figure 5: AMPKβ1 myristoylation and mitochondrial AMPK mediate mitochondrial removal. | Nature Communications

Figure 5: AMPKβ1 myristoylation and mitochondrial AMPK mediate mitochondrial removal.

From: Myristoylation confers noncanonical AMPK functions in autophagy selectivity and mitochondrial surveillance

Figure 5

(a) Subcellular localization of GFP-AMPKβ1 (β1) and the myristoylation defective mutant AMPKβ1G2A (β1G2A) and co-localization with mCherry-LC3 in the presence and absence of bafilomycin A1 (100 nM; 2 h). Scale bar, 20 μm. (b) Western analysis of TOM20 levels in H23 cells transfected with GFP-AMPKβ1 or GFP-AMPKβ1G2A treated with CCCP for 4 h. ERK2 was blotted for loading control. (c) The generation of mitochondrion targeting AMPKβ1 (Mito-β1-GFP) by N terminally tagging AMPKβ1-GFP with the mitochondrial localization signal (Mito) from TOM20. (d,e) H23 cells (d) and MEFs (e) transfected with Mito-β1-GFP with mitochondria stained with MitoTracker Red (MTR). Circled cells show typical mitochondrial alterations (loss of peripheral portion of the mitochondria and disruption of the mitochondrial network) that are not found in ATG5−/− cells. Scale bar, 20 μm.

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