Figure 2: The loss of H1KD GSCs is due to premature differentiation triggered by bam upregulation. | Nature Communications

Figure 2: The loss of H1KD GSCs is due to premature differentiation triggered by bam upregulation.

From: Histone H1-mediated epigenetic regulation controls germline stem cell self-renewal by modulating H4K16 acetylation

Figure 2

(a–c) bamP-GFP expression (green) is repressed in GSCs of control flies (a), but expands to the GSCs in nos-GAL4>H1KD germaria (arrowhead in b). Note that the bamP-GFP-positive germline cells (arrow) neighbouring the cap cells (red nuclear lamina) in c share a branched fusome (red), indicating that premature differentiation of the GSCs occurs in H1KD germaria. Arrowheads in a point to GSCs. (d) qRT–PCR results show that the transcription of bam in nos-GAL4>H1KD ovaries has increased to 1.5-fold compared with that in the control (n=3, mean±s.d.). (e) Bam (red) is increased in the GFP-marked H1KD GSCs (broken circles) compared with that in the GFP-negative control GSCs (circles) 7 days ACI. (f) Quantification of results in e showing that the relative Bam intensity in GFP-marked H1KD GSCs is about twofold higher than that in GFP-negative control GSCs (n=5, mean±s.d.). The intensity of Bam in control GSCs is set to 1. Data are evaluated with Student’s t-test. (g,h) The bamΔ86 heterozygous mutation (h) can suppress GSC loss in nos-GAL4>H1KD females (g). (i) Quantification of results in g,h showing that the bamΔ86 heterozygous mutation can rescue the GSC reduction in H1KD germaria. n=61 and n=25 for nos-GAL4>H1KD and nos-GAL4>H1KD; bam+/− groups, respectively. Scale bars, 10 μm. DAPI, 4,6-diamidino-2-phenylindole.

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