Supplementary Figure 4: H3K27ac HiChIP biological replicates from primary sorted T cells are highly reproducible. | Nature Genetics

Supplementary Figure 4: H3K27ac HiChIP biological replicates from primary sorted T cells are highly reproducible.

From: Enhancer connectome in primary human cells identifies target genes of disease-associated DNA elements

Supplementary Figure 4

(a) FACS strategy for naive, TH17, and Treg cells starting from human peripheral blood. Post-sort validation was used to ensure purity of T cell subtypes. Number represents the percentage of cells within that gate. (b) KR balanced interaction map of T cell subtype biological replicates. Each replicate corresponds to one side of the interaction map, separated by the diagonal. (c) Read support reproducibility of loops between H3K27ac HiChIP biological replicates in naive, TH17, and Treg cells. (d) Aggregate peak analysis of loops in naive, TH17, and Treg H3K27ac HiChIP libraries.

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