Supplementary Figure 5: Clonal reprogramming analysis of different cell types.
From: Small molecules facilitate rapid and synchronous iPSC generation
(a) Intra-well heterogeneity of OCT4-GFP expression in proB cells during reprogramming. Each time point represents analysis of a 96-well plate. (b) Intra-well heterogeneity of OCT4-GFP expression in hematopoietic stem cells (HSCs) during reprogramming. Each time point represents analysis of a 96-well plate. (c) Schematic of clonal reprogramming assay in GMPs using OCT4-GFP expression after doxycycline withdrawal as readout. (d) Quantification of clonal iPSCs generated after administering doxycycline with or without AGi for the indicated times. Doxycycline was withdrawn for at least 3 days prior to analysis to ensure that iPSC colonies were stable. Box and whisker plots were used in which the band in the middle of the box represents the median; the top and bottom of the box represent the first and third quartiles. The whiskers represent the lowest and highest datum within 1.5-fold of the inter-quartile ranges.
