Figure 3: PLT5/7 transcription factors control species-specific leaf shape.
From: The Cardamine hirsuta genome offers insight into the evolution of morphological diversity
a, Venn diagram showing the intersection of shoot meristem-expressed genes and transcription factor-encoding genes that are expressed more than twofold higher in C. hirsuta than in A. thaliana leaves. b,c, In situ hybridization of ChPLT5 (b) and ChPLT7 (c) expression in transverse and longitudinal (shown in inset) sections through C. hirsuta shoot apices. Arrows indicate signal in the shoot apical meristem and leaflets. Scale bars, 100 µm. d–g, Rosette leaf 5 (d,f) and cauline leaves (e,g) of C. hirsuta wild type (WT) (d,e) and 35S::amirChPLT5,7 (f,g). h, Quantification of leaflet number in rosette leaf 5 of wild type and 35S::amirChPLT5,7. Error bars show standard error of leaflet number. ***, regression of leaflet number on genotype (P < 0.001). i,j, Representative rosette leaves of A. thaliana vector-only control (i) and CUC2::ChPLT7 (j). N = 20/20 (i) and 4/27 (j) independent transgenic lines with similar phenotypes. Scale bars, 1 cm.
