Supplementary Figure 4: Ensemble FRET measurements of KirBac1.1 mutants labeled at different structural motifs in liposomes with or without PIP2.
From: Structural dynamics of potassium-channel gating revealed by single-molecule FRET
KirBac1.1 mutants were labeled with EDANS/DABCYL-plus (A) or AlexaFluor-488/QSY7 FRET pair (B) and reconstituted into liposomes (POPE:POPG = 3:1) with or without 1% PIP2. Ensemble FRET measurements were performed following the protocols as described previously (Wang, S. et al., 2012, Nature communications 3, 617). The data were presented as mean± se, n = 6 (*p<0.05, **p<0.01).
