Fig. 1

Chromosome transfer technology and the top-down HAC/MAC. a Chromosome transfer technology: MMCT. Donor CHO, A9, and DT40 cells are exposed to a microtubule inhibitor leading to micronucleation. Microcells are then purified and fused to target cells. Microcell hybrids, target cells containing the desired chromosome, can be obtained. b Construction of the top-down HAC/MAC. HACs and MACs are stable in humans and mice, respectively