Fig. 4: MD-1 induces the formation of NETs. | Experimental & Molecular Medicine

Fig. 4: MD-1 induces the formation of NETs.

From: Neutrophil extracellular traps mediate the crosstalk between plaque microenvironment and unstable carotid plaque formation

Fig. 4

a Isolation and validation of neutrophils (Giemsa staining, Trypan blue staining, and cell counter, scale bar = 20 μm). b Hochest staining and ELISA were used to quantify the concentration and time course of MD-1-induced NETs. c NETs were quantified in the negative control group, the MD-1 (75 ng/ml) group, and the two positive control groups. d Co-IP revealed that MD-1 and TLR4 coexisted on neutrophils. e The interaction between MD-1 and TLR4 in peripheral blood neutrophils from carotid plaque patients and peripheral blood neutrophils from healthy volunteers induced under different conditions was analyzed by Duolink PLA (scale bar = 100 μm). f Western blot analysis showed that neutrophils induced with MD-1 upregulated TLR4 expression and increased the phosphorylation of IRAK1/IRAK4, p38 MAPK, and ERK1/2 in a time-dependent manner (from 0 to 60 min). t total value; p phosphorylation value. β-Actin was used as an internal control.

Back to article page