Fig. 8: Downregulation of YTHDF1 in MASLD reduces the mRNA stability of Hint2. | Experimental & Molecular Medicine

Fig. 8: Downregulation of YTHDF1 in MASLD reduces the mRNA stability of Hint2.

From: Histidine triad nucleotide-binding protein 2 attenuates metabolic dysfunction-associated steatotic liver disease through NAD+-dependent sirtuin-3 activation

Fig. 8

a Protein and mRNA expression of HINT2 in siYTHDF1 HepG2 cells and siYthdf1 primary hepatocytes. b Protein and mRNA expression of HINT2 in ovYTHDF1 HepG2 cells and ovYthdf1 AML12 cells. c The strength of Hint2 mRNA binding to YTHDF1 in hepatocytes. d The strength of Hint2 mRNA binding to YTHDF1 in Ythdf1-silenced hepatocytes. e Hint2 mRNA stabilities after actinomycin D treatment in Ythdf1-silenced AML12 cells. f Hint2 mRNA stabilities after actinomycin D treatment in Ythdf1-overexpressing AML12 cells. g, h Protein and mRNA expression of YTHDF1 in the livers of HFD- (g) and WDSW- (h) fed mice. i, j Protein and mRNA expression of YTHDF1 in FFA-stimulated HepG2 cells (i) and AML12 cells (j). k Oil Red staining and intracellular TG contents of HepG2 cells overexpressing YTHDF1 under FFA stimulation. l Oil Red staining and intracellular TG contents of HepG2 cells silencing YTHDF1 under FFA stimulation. Data are presented as mean ± s.d. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 (Student’s t-test).

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