Fig. 5
From: Primary cilia support cartilage regeneration after injury
Disruption cilia structure to block Hh signaling in Col2+ chondrocytes impairs growth plate development. a Representative images of Ift140fl/fl (CTRL) and Col2-cre; Ift140fl/fl (cKO) mice at indicated ages. Scale bar, 1 cm. b Representative micro-CT images showing femur and tibia from Ift140fl/fl (CTRL) and Col2-cre; Ift140fl/fl (cKO) mice at indicated ages. Scale bar, 1 mm. c Representative H&E staining of distal-femur growth plates showing growth plate morphology from Ift140fl/fl (CTRL) and Col2-cre; Ift140fl/fl (cKO) mice at indicated ages. SOC, secondary ossification center. POC, primary ossification center Scale bar, 100 μm. d 1-week-old Ift140fl/fl (CTRL) and Col2-cre; Ift140fl/fl (cKO) distal-femur growth plates with EdU administration shortly before analysis. Scale bars, 200 μm. e Immunofluorescence staining visualizes primary cilia in distal-femur growth plates from one week old mice. Ace α-tubulin stains axoneme. Scale bar, 10 μm. f Immunofluorescence staining of IHH in distal-femur growth plates. Arrows indicate the IHH in pre-hypertrophic zone. Scale bar, 200 μm
