Fig. 2

Intermittent PTH regulated the gene network of Wnt/β-catenin signaling in OCCM‐30 cells. a Relative mRNA expression of Wnt-related genes. The mRNA expression of β-catenin, Axin2 and APC were all decreased upon exposure to PTH treatment. n = 6; *P < 0.05, **P < 0.01, ***P < 0.001. b Western blot analyses revealed that the expression of β-catenin, Axin2 and APC was decreased with intermittent PTH application. c Quantification was performed to show the protein changes of β-catenin, Axin2 and APC. n = 3; *P < 0.05, **P < 0.01, ***P < 0.001. d Linear fluorescent quantification was performed to show the distribution of β-catenin cross the cell body in both groups. e Immunofluorescence staining of β-catenin. The β-catenin labeling was impaired at the presence of intermittent PTH. Scale bar: 50 µm