Fig. 4

Identification of Wnt-related lncRNA LITTIP under intermittent PTH. a Volcano plot displays global lncRNA expression in the control and intermittent PTH sets. Blue represents downregulated lncRNAs; red represents upregulated lncRNAs. b Construction of lncRNAs-mRNAs correlation network. Wnt signaling related mRNA LGR6 was represented as blue circular nodes and lncRNAs were represented as yellow circular nodes. c Targeted lncRNAs were identified by taking intersection of the Wnt-related lncRNAs and the lincRNAs. Two lincRNAs (lncRNA AK032137 and AK020949) were included. AK032137 was further investigated in this study and named LITTIP (LGR6 intergenic transcript under intermittent PTH). d Relative expression of LITTIP were decreased after 2–3 cycles of PTH treatment. n = 6; *P < 0.05, **P < 0.01. e Relative mRNA expression of LGR6 were decreased after 1–3 cycles of PTH treatment. n = 6; *P < 0.05, ***P < 0.001. f Western blotting and quantification showing the expression of LGR6 after 1–3 cycles of PTH treatment. n = 3; ***P < 0.001. g, h Cellular fractionation analyses showed that LITTIP was mainly located in the nucleus (g), and intermittent PTH decreased the expression of nuclear LITTIP (h). i Adenoviruses were used to overexpress LITTIP in OCCM-30 cells. Relative expression of LITTIP were remarkably increased. n = 6; ***P < 0.001. j Cellular fractionation analyses showed that adenoviruses increased the expression of nuclear LITTIP