Fig. 7

LncRNA LITTIP promotes expression of LGR6 via HnRNPK protein. a, b OCCM-30 cells were co-transfected with adenovirus of LITTIP and si-HnRNPK. Relative expression of LITTIP were remarkably increased in Ad + si-NC and Ad + si-HnRNPK groups (a). Relative mRNA expression of HnRNPK was decreased in NC + si-HnRNPK and Ad + si-HnRNPK groups (b). n = 6; **P < 0.01, ***P < 0.001. c, d Relative mRNA expression of LGR6 and β-catenin. The mRNA expression of LGR6 (c) and β-catenin (d) was upregulated by overexpression of LITTIP and decreased by si-HnRNPK. Downregulation of HnRNPK reversed the effects of LITTIP overexpression on mRNA expression of LGR6 and β-catenin. n = 6; *P < 0.05, ***P < 0.001. e Western blotting was performed to show relative protein expression of HnRNPK, LGR6, β-catenin and nucleus β-catenin. The expression of LGR6, β-catenin and nucleus β-catenin was enhanced by adenovirus of LITTIP. Downregulation of HnRNPK reversed the increased expression of LGR6, β-catenin and nucleus β-catenin by LITTIP overexpression. f Immunofluorescence staining of LGR6. Scale bar: 50 µm. g Linear fluorescent quantification was performed to show the cellular distribution of LGR6 in OCCM-30 cells. The LGR6 labeling was enhanced by adenovirus of LITTIP and this effect was impaired when HnRNPK was downregulated by siRNA