Fig. 7
From: Sema3A secreted by sensory nerve induces bone formation under mechanical loads
Sema3A promotes mitochondrial fusion in hPDCLs under mechanical overloads. a Tom20 and phalloidin staining demonstrated that mitochondria were distributed as a network along with F-actin in control group. Mechanical overload led to mitochondrial fragmentation and disassociation from F-actin. Sema3A treatment alleviated the mechanical overload-induced mitochondrial fragmentation, restoring the mitochondria network accompanied by F-actin. The ROCK2 inhibitor Y27632 disrupted the cytoskeleton, leading to the disintegration of the mitochondrial network in hPDLCs. b Western Blot revealed that Sema3A promoted the expression of Mfn2 protein in hPDLCs under mechanical overload, whereas the ROCK2 inhibitor Y27632 inhibits Mfn2 protein expression. c Immunofluorescence revealed that Sema3A restored the decreased Mfn2 expression and inhibited the Drp1 expression, and the restorative effect was attenuated by the ROCK2 inhibitor Y27632. d The Western Blot revealed that MIF8 exhibited little effects on the expression of ROCK2 protein, while effectively inhibit the expression of Runx2 protein in hPDLCs. Even in the presence of Sema3A, MFI8 retained the inhibitory effect on Runx2 expression. e, f Quantification of the Runx2 (e) and ROCK2 (f) protein in (d) mean ± SD, and Two-tailed Student’s t-test was used for comparison. *P < 0.05; **P < 0.01; ***P < 0.001
