Fig. 3 | Laboratory Investigation

Fig. 3

From: Loss of Fezf2 promotes malignant progression of bladder cancer by regulating the NF-κB signaling pathway

Fig. 3

Downregulation of Fezf2 promotes aggressiveness in bladder cancer cells. a Western blotting analysis of Fezf2 expression in EJ and T24 cells with stable silencing of Fezf2; α-tubulin was used as a loading control. b Representative micrographs (left panel) and quantification (right panel) of EdU incorporation in indicated bladder cancer cells. DAPI was used as a DNA/nuclear stain. c Representative images of CAM blood vessels stimulated with conditioned medium from indicated cells. d Representative pictures (left panel) and quantification (right panel) of invading cells were analyzed using a transwell Matrigel assay. e Flow cytometric analysis showing percentages of Fezf2-knockdown cells at different phases of the cell cycle. f Representative images (left panel) and quantification (right panel) of HUVECs cultured on matrigel-coated plates with conditioned medium from Fezf2-knockdown cells. Bar graphs show statistical analysis of three independent experiments (*P < 0.05)

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