Fig. 5
Fezf2 regulates the NF-κB signaling pathway in bladder cancer. a GSEA plot showing Fezf2 expression is negatively correlated with NF-κB-activated gene signatures. b Analysis of luciferase reporter activity in indicated cells after transfection with pNF-κB–luc plasmids or control-luciferase plasmid. c Real-time PCR analysis of NF-κB signaling pathway downstream gene expression in indicated cells. d Western blotting analysis of expression levels of indicated proteins in indicated cells. α-tubulin was used as a loading control. e Quantification of colony numbers in anchorage-independent growth assay. Colonies larger than 0.1 mm in diameter were scored. f Quantification (right panel) of invading cells analyzed with a transwell Matrigel assay. g Quantification of tubule formation by HUVECs cultured in Matrigel-coated plates with conditioned media from bladder cancer cells transfected with the vector IκBα-mut, or treated with the NF-κB inhibitor JSH-23. Each bar represents the mean ± SD of three independent experiments. *P < 0.05
