Fig. 5
From: High-mobility group box 1 induces endoplasmic reticulum stress and activates hepatic stellate cells
Signaling pathways downstream of HMGB1 are involved in liver fibrosis and ER stress. a TLR4, TLR2 and RAGE receptors were shown by western blot in TAA-induced fibrosis models (n = 2) in each group. b The protein levels of α-SMA and collagen-1 in LX-2 cells treated with anti-TLR4 antibody (a-TLR), goat IgG (IgG), rhHMGB1, rhHMGB1 with a-TLR or rhHMGB1 with IgG were determined by western blot. c, d The protein levels of α-SMA and collagen-1 in LX-2 and HSC-T6 cells treated with anti-RAGE antibody (a-RAGE), goat IgG (IgG), rhHMGB1, rhHMGB1 with a-RAGE or rhHMGB1 with IgG were determined by western blot. e The protein levels of PERK, IRE1α and GRP78 in LX-2 cells treated with anti-TLR4 antibody (a-TLR), goat IgG (IgG), rhHMGB1, rhHMGB1 with a-TLR or rhHMGB1 with IgG were determined by western blot. f, g The protein levels of PERK, IRE1α and GRP78 in LX-2 and HSC-T6 cells treated with anti-RAGE antibody (a-RAGE), goat IgG (IgG), rhHMGB1, rhHMGB1 with a-RAGE or rhHMGB1 with IgG were determined by western blot. h, i LX-2 and HSC-T6 cells were treated with rhHMGB1 (100 ng/ml) or 4-PBA or rhHMGB1+4-PBA to show the protein expression of IL-1β and IL-18
