Fig. 6
From: High-mobility group box 1 induces endoplasmic reticulum stress and activates hepatic stellate cells
The inhibition of HMGB1 reduces liver fibrosis in vivo. a Overview of the procedure. Hepatic fibrosis was induced in rats by intraperitoneal injection of TAA two times/week for 6 weeks. Subsequently, each animal received 5 × 109 PFUs of HMGB1 shRNA (AdshHMGB1) or control adenovirus vector (AdshNC) via the tail vein, one time/week and was repetitively administered TAA for 2 weeks. b H&E and Masson’s trichrome staining were used to examine the pathological alterations and collagen deposition in AdshNC group and AdshHMGB1 group. c Semiquantitative analysis of Masson’s trichrome staining in the fibrotic livers from AdshNC- or AdshHMGB1-treated rats made by the Image J (n = 8 rats in each group). d The expression of collagen-1 and α-SMA in the fibrotic livers was analyzed by immunohistochemistry. e Perk, IRE1α and grp78 in the fibrotic livers was analyzed by immunohistochemistry. f The protein levels of PERK, IRE1α, grp78, collagen-1, α-SMA and HMGB1 in the livers were detected by western blot (n = 3) respectively. GAPDH was used as an endogenous control. g Serum biochemical parameters, ALT and AST, were analyzed from AdshNC- or AdshHMGB1-treated rats (n = 8 rats in each group). **P < 0.01, ***P < 0.0001
