Fig. 6 | Laboratory Investigation

Fig. 6

From: Surfactant dysfunction and alveolar collapse are linked with fibrotic septal wall remodeling in the TGF-β1-induced mouse model of pulmonary fibrosis

Fig. 6

3D analysis of an AE2 cell showing no contact to alveolar airspace in a 2D section (d). Correlative light microscopy with SEM array tomography was used to locate and reconstruct a region of interest in 3D. About 665 sections were analyzed by light microscopy and the identified ROI was scanned with SEM at high resolution (a: sections on microscope slide; b–d ascending magnifications and resolutions of ROI). Image D: AE2 cell of interest in 2D (asterisk; scale bar: 5 µm). Model of reconstructed AE2 cell is depicted with tissue context in three z-layers (e); asterisk indicates the same position in d and e; baso-lateral plasma membrane (beige), apical plasma membrane (green), nucleus (blue), lamellar bodies, and lamellar-body like structures (red). The AE2 cell had no exposure to the air space—only contact with edema fluid was existent. The AE2 cell model is shown in different orientations (f). Abbreviations: air: distal airspace, cap: capillary lumen, AE2: alveolar epithelial type 2 cell, ed: alveolar edema fluid

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