Fig. 3
From: Micheliolide ameliorates renal fibrosis by suppressing the Mtdh/BMP/MAPK pathway
DMAMCL protects kidney from fibrosis in the IRI mice. a Representative micrographs of Masson’s trichrome staining and IHC staining for α-SMA, fibronectin, and E-cadherin in the injured kidneys. Scale bar in Masson’s trichrome staining is 100 μm; in IHC staining is 50 μm. b Quantification of renal tubular interstitial fibrotic score. ****P < 0.0001 compared with the sham group; #P < 0.05 compared with the vehicle group. c Serum creatine level in the IRI mice. ****P < 0.0001 compared with the sham group; ##P < 0.01 compared with the vehicle group. d Serum urea level in the IRI mice. ****P < 0.0001 compared with the sham group; ###P < 0.001 compared with the vehicle group. e Representative bands from Western blot analyses of the levels of the α-SMA, fibronectin, and E-cadherin proteins in kidney tissues from the IRI mice. f Relative protein levels as determined by the Western blot assay (e). ***P < 0.001 compared with the sham group; ##P < 0.01 compared with the vehicle group. n = 6 mice per group, all the data are presented as the mean ± SEM of at least three independent experiments
