Fig. 5

ALK fusion variant distribution across any two parameters from the second derivative curve. Twenty ALK fusion variant standards (empty circles) were separated in two-dimensions, including monotonic (a) and non-monotonic variants (b). ALK fusion positive FFPE samples (P1–P7, gray solid and empty squares) are also shown. P1 and P2 were close to variant E6a/b:A20, P3 was close to variant KL9:A20, and P4–P7 were close to variant E13:A20. Horizontal and vertical bars indicate the 95% confidential intervals from repeated samples