Fig. 4: miR-199a-5p-modified ADSCs package miR-199a-5p into secreted extracellular vesicles (EVs). | Laboratory Investigation

Fig. 4: miR-199a-5p-modified ADSCs package miR-199a-5p into secreted extracellular vesicles (EVs).

From: Extracellular vesicles derived from miR-199a-5p-modified adipose-derived mesenchymal stem cells alleviate immune thrombocytopenia by inhibiting T helper 17 differentiation

Fig. 4

A The expression levels of surface markers in ADSCs isolated from mice were analyzed using flow cytometry. B After culturing in adipogenesis differentiation medium for 14 days, the adipogenesis differentiation of ADSCs was evaluated by Oil Red staining. Scale bar = 100 μm. C After culturing in osteogenesis differentiation medium for 21 days, the osteogenesis differentiation of ADSCs was evaluated by Alizarin Red S staining. Scale bar = 200 μm. D EVs isolated from ADSCs transfected with agomir NC (NC-EVs) and isolated from ADSCs transfected with miR-199a-5p agomir (miR-199a-5p-EVs) were visualized using Transmission Electron Microscope (TEM). Scale bar = 100 nm. E The expression levels of CD63 and CD81 in miR-199a-5p-EVs and NC-EVs were measured by western blot. Calnexin was used as a negative control. F miR-199a-5p expression was measured by qRT-PCR in miR-199a-5p-EVs and NC-EVs. ***P < 0.001 vs. NC-EVs.

Back to article page