Fig. 6: AOPPs downregulated CYP1A2 and CYP3A4 expression via the NF-κB pathway. | Laboratory Investigation

Fig. 6: AOPPs downregulated CYP1A2 and CYP3A4 expression via the NF-κB pathway.

From: Advanced oxidation protein products downregulate CYP1A2 and CYP3A4 expression and activity via the NF-κB-mediated signaling pathway in vitro and in vivo

Fig. 6

NF-κB-dependent firefly luciferase reporter gene was transfected into HepG2 or L-02 cells, and the reporter gene expression (A). HepG2 or L-02 cells were treated with AOPPs (200 µg/ml) for 48 h and cocultured with BAY-117082 (B) and PDTC (C) to restore the downregulated expression levels of CYP1A2 and CYP3A4. Protein expression levels of CYP1A2 and CYP3A4 were quantified by ImageJ software (D, E). Each experiment was performed with a different isolate. Data are presented as mean ± SD; *p < 0.05 compared with the CON group; #p < 0.05 compared with the group without BAY-117082 or PDTC. Data were normalized to GAPDH.

Back to article page