Fig. 7: AOPPs downregulated CYP1A2 and CYP3A4 expression via the inflammatory cytokine pathway. | Laboratory Investigation

Fig. 7: AOPPs downregulated CYP1A2 and CYP3A4 expression via the inflammatory cytokine pathway.

From: Advanced oxidation protein products downregulate CYP1A2 and CYP3A4 expression and activity via the NF-κB-mediated signaling pathway in vitro and in vivo

Fig. 7

AOPPs increased the plasma concentrations of IL-6 and TNF-α in the sham and 5/6 nx groups (A). AOPPs increased the IL-6 and TNF-α concentrations in rat macrophages (B). HepG2 and L-02 cells treated with various concentrations of IL-6 (C) or TNF-α (D) for 48 h exhibited dose-dependent decreases in the protein expression levels of CYP1A2 and CYP3A4, as evaluated by western blotting. Protein expression levels of CYP1A2 and CYP3A4 levels were quantified by ImageJ software (E, F). Each experiment was performed with a different isolate. Data are presented as mean ± SD; *p < 0.05 compared with the CON group, #p < 0.05 compared with the sham group. Data were normalized to GAPDH.

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