Fig. 5: BG affects Cx43 expression and ROS levels. | Laboratory Investigation

Fig. 5: BG affects Cx43 expression and ROS levels.

From: Bioglass promotes wound healing by inhibiting endothelial cell pyroptosis through regulation of the connexin 43/reactive oxygen species (ROS) signaling pathway

Fig. 5

a Western blotting was conducted to detect the expression of Cx43 in different groups at day 7 after surgery. Six fields of view were randomly selected for each sample, and ~200–300 cells were observed. b Quantitative analysis of the protein expression of Cx43 compared with the normal group. *P < 0.05 vs. NC group, n = 3. c, d Cx43/CD31 double staining was performed to assay Cx43 expression in endothelial cells from the wound edge on the 7th day after surgery. Scale bar = 50 μm. ***P < 0.001 vs. NC group, n = 6. e, f ROS levels in the wound area were measured by using a ROS probe (dihydroethidium, DHE) with different treatments. Scale bar = 250, 75 μm. ***P < 0.001 vs. NC group, n = 3.

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