Fig. 7: BG rescues LPS-ATP-induced pyroptosis in HUVECs. | Laboratory Investigation

Fig. 7: BG rescues LPS-ATP-induced pyroptosis in HUVECs.

From: Bioglass promotes wound healing by inhibiting endothelial cell pyroptosis through regulation of the connexin 43/reactive oxygen species (ROS) signaling pathway

Fig. 7

a The effect of BG ion extract diluted with serum-free ECM in different ratios from 1/10 to 1/500 on cell proliferation, n = 6. b Effects of various concentrations of BG on proliferation of HUVECs treated with LPS-ATP. ***P < 0.001 vs. LPS-ATP group, n = 6. c LDH release in HUVECs treated with LPS-ATP plus different concentrations of BG was assayed. **P < 0.01 and ***P < 0.001 vs. LPS-ATP group, n = 3. d, e Hoechst/PI staining was performed to detect the percentage of PI-positive cells in HUVECs. Scale bar = 20 μm. ***P < 0.001 vs. LPS-ATP group, n = 6. f Western blotting was conducted to analyze the expression of pyroptosis-related proteins in LPS-ATP-stimulated HUVECs treated with 1/200 of BG. g–m Quantitative analysis of the expression of pyroptosis-related proteins in each group compared with control. **P < 0.01 vs. LPS-ATP group, n = 3.

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