Fig. 2: Knockdown of miR-211-3p suppressed Ang II-induced cardiomyocyte hypertrophy in H9C2 cells. | Laboratory Investigation

Fig. 2: Knockdown of miR-211-3p suppressed Ang II-induced cardiomyocyte hypertrophy in H9C2 cells.

From: LncRNA TINCR improves cardiac hypertrophy by regulating the miR-211-3p-VEGFB-SDF-1α-CXCR4 pathway

Fig. 2

A Cell size analysis in untreated and Ang II-treated cells (n = 3). B The relative mRNA expression of ANP, BNP, β-MHC, and Col1 (n = 3). C Western blot analysis of ANP, BNP, β-MHC, and Col1 (n = 3). GAPDH was used as a loading and normalization control. D The relative expression of TINCR and miR-211-3p in H9C2 cells treated with Ang II and untreated cells (n = 3). E The relative expression of miR-211-3p in H9C2 cells transfected with miR-211-3p inhibitor or inhibitor NC (n = 3). F Cell size analysis in Ang-induced H9C2 cells transfected with miR-211-3p inhibitor or inhibitor NC. G The relative mRNA expression of ANP, BNP, β-MHC, and Col1 in H9C2 cells with the indicated treatment by RT-qPCR (n = 3). H Western blot analysis of ANP, BNP, β-MHC, and Col1 in H9C2 cells with the indicated treatment (n = 3). GAPDH was used as a loading and normalization control. Data were from at least three independent assays. *P < 0.05, **P < 0.01, and ***P < 0.001.

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