Fig. 3: LINC01963 silencing reduced PC3-DR cell survival while LINC01963 overexpression increased PC3-DR cell survival. | Laboratory Investigation

Fig. 3: LINC01963 silencing reduced PC3-DR cell survival while LINC01963 overexpression increased PC3-DR cell survival.

From: Silencing of LINC01963 enhances the chemosensitivity of prostate cancer cells to docetaxel by targeting the miR-216b-5p/TrkB axis

Fig. 3

PC3-DR cells were treated with 40 nmol/L DTX in those studies. a Proliferation of PC3-DR cells was analyzed by MTS. b, d PC3-DR cell apoptosis was analyzed by flow cytometry. Apoptosis is shown as the mean ± SD (b), and a representative image of apoptosis is shown (d). c, e The cell cycle of PC3-DR was analyzed by flow cytometry. G1 phase distribution is shown as the mean ± standard deviation of three technical replicates (c) and a representative image of the cell cycle is shown (e). f Tumor tissues in nude mice were excised and photographed 30 days after xenograft study. g, h Tumor volumes and weights were detected. i The degree of lung metastasis in nude mice was evaluated by an in vivo imaging system. ***P < 0.001 vs. NC group.

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