Fig. 5: miR-216b-5p was bound by LINC01963. | Laboratory Investigation

Fig. 5: miR-216b-5p was bound by LINC01963.

From: Silencing of LINC01963 enhances the chemosensitivity of prostate cancer cells to docetaxel by targeting the miR-216b-5p/TrkB axis

Fig. 5

a StarBase 3.0 and LncBase 2.0 analysis found that 11 common miRNAs had a promising binding site with LINC01963. b miRNA expression was measured by qRT-PCR in PC3 and PC3-DR cells. PC3 and PC3-DR cells were not treated with DTX in those studies. c miR-216b-5p expression in the Blank, NC, ov-LINC01963, sh-LINC01963 groups was measured by qRT-PCR in PC3 and PC3-DR cells. PC3 cells were treated with 4 nmol/L DTX and PC3-DR cells were treated with 40 nmol/L DTX. d The binding sites between LINC01963 and miR-216b-5p are shown. e The binding sites between LINC01963 and miR-216b-5p were determined using a dual luciferase assay. f The binding sites between LINC01963 and miR-216b-5p were determined using an AGO-RIPA assay. g The binding sites between LINC01963 and miR-216b-5p were determined using a pull-down assay. Data are shown as the mean ± standard deviation of three technical replicates. ***P < 0.001.

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