Fig. 7: NTRK2 is the target gene of miR-216b-5p. | Laboratory Investigation

Fig. 7: NTRK2 is the target gene of miR-216b-5p.

From: Silencing of LINC01963 enhances the chemosensitivity of prostate cancer cells to docetaxel by targeting the miR-216b-5p/TrkB axis

Fig. 7

PC3-DR cells were treated with 40 nmol/L DTX and PC3 cells were treated with 4 nmol/L DTX in those studies. a The binding sites between the 3′-UTR of NTRK2 and miR-216b-5p were analyzed by Targetscan 7.2 and miRWalk. b The binding sites between the 3′-UTR of NTRK2 and miR-216b-5p were determined using a dual luciferase assay. c TrkB protein level was analyzed by western blot assay in PC3 cells treated with 4 nmol/L DTX and PC3-DR cells treated with 40 nmol/L DTX. ***P < 0.001 vs. Blank group. d TrkB protein level was analyzed by western blot assay in PC3-DR cells treated with 40 nmol/L DTX. ***P < 0.001 vs. Blank group. e NTRK2 mRNA expression was measured by qRT-PCR in PC3 cells treated with 4 nmol/L DTX and PC3-DR cells treated with 40 nmol/L DTX. Data are shown as the mean ± standard deviation of three technical replicates.

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