Fig. 4: Positive feedback of HIF-1α and CD146 in epithelial cells. | Laboratory Investigation

Fig. 4: Positive feedback of HIF-1α and CD146 in epithelial cells.

From: The CD146-HIF-1α axis regulates epithelial cell migration and alveolar maturation in a mouse model of bronchopulmonary dysplasia

Fig. 4

A Representative immunofluorescence imaging of CD146 in WT or CD146 KO primary alveolar epithelial cells. Scale bars, 200 μm. B Western blot analysis of HIF-1α and CD146 expression in MLE-12 cells treated with a HIF-1α expression plasmid. C, D qRT–PCR and Western blot analysis of HIF-1α and CD146 expression in MLE-12 cells treated with a CD146 expression plasmid. E Western blot analysis of CD146, HIF-1α, and P65 expression in primary alveolar epithelial cells. All experiments were repeated at least 3 times. *P < 0.05; **P < 0.01; ***P < 0.001.

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