Fig. 5: Increased HIF-1α and CD146 in the early stage of BPD. | Laboratory Investigation

Fig. 5: Increased HIF-1α and CD146 in the early stage of BPD.

From: The CD146-HIF-1α axis regulates epithelial cell migration and alveolar maturation in a mouse model of bronchopulmonary dysplasia

Fig. 5

Newborn mice were exposed to 65% hyperoxia until the 5th day, when the puppies exposed to oxygen returned to indoor air to establish the BPD model. A Flow chart showing the BPD model. B Body weight was recorded at 6, 9, 12, and 14 days after birth. C–E Lung morphometry was analyzed by H&E staining of tissue samples from surviving pups at day 14 post birth. Scale bars = 100 μm. F Determination of respiratory resistance (Rrs) and compliance (Crs) in the lungs of BPD and normal mice by the finepointerc system. G Methacholine dose-dependent changes in Rrs and Crs were observed in BPD and control mice. H, I qRT–PCR and Western blot analysis of HIF-1α and CD146 expression in pulmonary tissues at day 14 post birth. J, K Representative results of immunohistochemical analysis of CD146 in pulmonary tissues at day 14 post birth. Five mice were included in the control and BPD groups at each time point. All experiments were repeated at least 3 times. Scale bars, 100 μm. *P < 0.05; **P < 0.01; ***P < 0.001.

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