Fig. 7: CD146 deficiency relieved the disease severity of BPD. | Laboratory Investigation

Fig. 7: CD146 deficiency relieved the disease severity of BPD.

From: The CD146-HIF-1α axis regulates epithelial cell migration and alveolar maturation in a mouse model of bronchopulmonary dysplasia

Fig. 7

Newborn mice were exposed to 65% hyperoxia until the 5th day, when the puppies exposed to oxygen returned to indoor air to establish the BPD model. A–C Lung morphometry was analyzed by H&E staining of tissue samples from surviving pups at day 14 post birth. Scale bar, 100 μm. D Determination of respiratory resistance (Rrs) and compliance (Crs) in the lungs of BPD mice by the finepointerc system. E Methacholine dose-dependent changes in Rrs and Crs were observed in BPD mice. F HIF-1α and CD146 protein expression in pulmonary tissues at day 14 post birth. G Representative results of immunohistochemical analysis of HIF-1α in pulmonary tissues at day 14 post birth. Scale bars, 200 μm. At least 3 mice were included in the control and BPD groups at each time point. All experiments were repeated at least 3 times. *P < 0.05; **P < 0.01; ***P < 0.001.

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