Fig. 6: Exos-HOTAIRM1 contributes to extracellular matrix remodeling in IPF mice through the miR-30d-3p/YY1/HSF1 axis. | Laboratory Investigation

Fig. 6: Exos-HOTAIRM1 contributes to extracellular matrix remodeling in IPF mice through the miR-30d-3p/YY1/HSF1 axis.

From: Exosomes derived from hypoxia-induced alveolar epithelial cells stimulate interstitial pulmonary fibrosis through a HOTAIRM1-dependent mechanism

Fig. 6

A RT–qPCR detection of HOTAIRM1, YY1, HSF1 and miR-30d-3p expression in the lung tissue of IPF mice and those treated with Exos-oe-HOTAIRM1 + sh-NC or Exos-oe-HOTAIRM1 + sh-HSF1. B Western blot analysis of α-SMA, collagen I, and fibronectin proteins in lung tissues of IPF mice and those treated with Exos-oe-HOTAIRM1 + sh-NC or Exos-oe-HOTAIRM1 + sh-HSF1. C Level of hydroxyproline in the lung tissue of IPF mice and those treated with Exos-oe-HOTAIRM1 + sh-NC or Exos-oe-HOTAIRM1 + sh-HSF1. D Lung compliance and resistance in the lung tissue of IPF mice and those treated with Exos-oe-HOTAIRM1 + sh-NC or Exos-oe-HOTAIRM1 + sh-HSF1. n = 6 for mice upon each treatment. The data are shown as the mean ± standard deviation. Data among multiple groups were assessed by one-way ANOVA with Tukey’s post-hoc tests. *p < 0.05, compared with normal mice or IPF mice treated with Exos-oe-NC + sh-NC. #p < 0.05, compared with IPF mice treated with Exos-oe-NC + sh-NC or Exos-oe-HOTAIRM1 + sh-NC. &p < 0.05, compared with IPF mice treated with Exos-oe-HOTAIRM1 + sh-NC.

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