Fig. 6: GTSE1 inhibition upregulated KLF4 in ccRCC. | Laboratory Investigation

Fig. 6: GTSE1 inhibition upregulated KLF4 in ccRCC.

From: GTSE1 promotes tumor growth and metastasis by attenuating of KLF4 expression in clear cell renal cell carcinoma

Fig. 6

A Volcano plot of differentially expressed genes (DEGs) was shown. B DEGs were annotated by KEGG pathway enrichment analysis. C TCGA database indicated GTSE1 was negatively associated with KLF4. D Knockdown GTSE1 upregulated KLF4 protein expression, validated by Western blot analysis in 786-O and Caki-1 cells. E In vitro proliferation assay was conducted in the form of rescue experiments. F, G In vitro transwell assay was performed in the form of rescue experiments. H, I Expression of the EMT-related proteins were assessed in Caki-1 Cells during the rescue assay. Graphs on the right show the quantification of western blot data from 3 independent experiments. **p < 0.01, ***p < 0.001.

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