Fig. 3: Elevated expression of miR-193a-5p suppresses NSCLC cell viability, migration, and invasion, but induces apoptosis. | Laboratory Investigation

Fig. 3: Elevated expression of miR-193a-5p suppresses NSCLC cell viability, migration, and invasion, but induces apoptosis.

From: Long non-coding RNA DLGAP1-AS1 modulates the development of non-small-cell lung cancer via the microRNA-193a-5p/DTL axis

Fig. 3

A The localization of lncRNA DLGAP1-AS1 in NSCLC cells predicted with the LncATLAS database. B The Venn diagram displaying the intersection of putative miRNAs that might bind to lncRNA DLGAP1-AS1 predicted by RNA22 and differentially expressed miRNAs from the microarray dataset GSE102286. C The expression of miR-193a-5p in NSCLC tissues from the microarray dataset GSE102286. D The expression of miR-193a-5p in NSCLC and adjacent normal tissues determined by RT-qPCR, n = 48. E The expression of miR-193a-5p in NCl-H1975 cells determined by RT-qPCR. F The viability of NCl-H1975 cells assessed by CCK-8 assay. G The apoptosis of NCl-H1975 cells measured by flow cytometry. H The NCl-H1975 cell migration and invasion measured by Transwell assay (× 200, scale bar = 50 µm); *p < 0.05, vs. adjacent normal tissues or NCl-H1975 cells transfected with mimic NC; **p < 0.01; Values were expressed as the mean ± standard deviation and analyzed with paired t-test or unpaired t-test. Values of cell viability were analyzed using ANOVA of repeated measurements, followed by a Bonferroni post hoc test for multiple comparisons; the experiment was repeated 3 independent times.

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