Fig. 4: LncRNA DLGAP1-AS1 upregulates DTL by binding to miR-193a-5p. | Laboratory Investigation

Fig. 4: LncRNA DLGAP1-AS1 upregulates DTL by binding to miR-193a-5p.

From: Long non-coding RNA DLGAP1-AS1 modulates the development of non-small-cell lung cancer via the microRNA-193a-5p/DTL axis

Fig. 4

A The binding site between miR-193a-5p and lncRNA DLGAP1-AS1. B The targeting relationship between lncRNA DLGAP1-AS1 and miR-193a-5p determined with dual luciferase reporter gene assay. C The enrichment of lncRNA DLGAP1-AS1 and miR-193a-5p assayed using RIP. D The enrichment of miR-193a-5p on DLGAP1-AS1 assessed with RNA pull-down. E The relative expression of miR-193a-5p in NSCLC cells determined with RT-qPCR. F The Venn diagram displaying the intersection of target genes of miR-193a-5p predicted by mirDIP, DIANA, RNA22, TargetScan and miRWalk and highly expressed genes from microarray dataset GSE19188. G The relative expression of KIF14 and DTL after miR-193a-5p expression was intervened in NSCLC cells determined with RT-qPCR. H The binding site between miR-193a-5p and DTL predicted with TargetScan. I The binding of miR-193a-5p to DTL confirmed by dual luciferase reporter gene assay. J The localization of DTL protein in NSCLC tissues and adjacent normal tissues detected by immunohistochemistry (× 200, scale bar = 50 µm). K The positive expression of DTL protein in NSCLC tissues and adjacent normal tissues determined by immunohistochemistry, n = 48. L Western blot analysis for the protein expression level of DTL in presence of miR-193a-5p mimic. M Western blot analysis for the protein expression level of DTL in presence of sh-DLGAP1-AS1. N Western blot analysis for the protein expression level of DTL in presence of pcDNA-DLGAP1-AS1 + miR-193a-5p mimic; *p < 0.05, vs. adjacent normal tissues or NCl-H1975 cells transfected with mimic NC; #p < 0.05, vs. the IgG group; &p < 0.05, vs. NCl-H1975 cells treated with Bio-probe NC or sh-NC; @p < 0.05, vs. NCl-H1975 cells transfected with pcDNA-NC; Values were expressed as the mean ± standard deviation; data between two groups were compared with unpaired t-test and data among multiple groups were compared with one-way ANOVA, followed by a Tukey multiple comparisons posttest; the experiment was repeated 3 independent times.

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