Fig. 5: Silencing lncRNA DLGAP1-AS1 suppresses NSCLC cell viability, migration, and invasion, but accelerates apoptosis via the miR-193a-5p/DTL axis. | Laboratory Investigation

Fig. 5: Silencing lncRNA DLGAP1-AS1 suppresses NSCLC cell viability, migration, and invasion, but accelerates apoptosis via the miR-193a-5p/DTL axis.

From: Long non-coding RNA DLGAP1-AS1 modulates the development of non-small-cell lung cancer via the microRNA-193a-5p/DTL axis

Fig. 5

A The expression of lncRNA DLGAP1-AS1, miR-193a-5p and DTL determined by RT-qPCR and Western blot analysis. B The viability of cells assessed by CCK-8 assay. C The cell migration measured by Transwell assay (× 200, scale bar = 50 µm). D The cell invasion measured by Transwell assay (× 200, scale bar = 50 µm). E The cell apoptosis measured by flow cytometry; *p < 0.05, vs. NCl-H1975 cells transfected with pcDNA-DLGAP1-AS1 + mimic NC; #p < 0.05, vs. NCl-H1975 cells transfected with sh-DLGAP1-AS1 + pcDNA-D-NC; &p < 0.05, vs. NCl-H1975 cells transfected with pcDNA-D-NC; Values were expressed as the mean ± standard deviation; data among multiple groups were compared with one-way ANOVA, followed by a Tukey multiple comparisons posttest; Values of cell viability were analyzed using ANOVA of repeated measurements, followed by a Bonferroni post hoc test for multiple comparisons; the experiment was repeated 3 independent times.

Back to article page