Fig. 1: GEMM mouse model characterization.

A Left: splenocytes isolated from 6 C57BL/6 (WT, black) or 7 Flt3-ITD+/–, Tet2+/–, Lys-Cre+/– (AML, red) mice were stained for live CD11b+ cells and evaluated by flow cytometry. Significance determined by Mann–Whitney t-test. Right: whole spleens isolated from 15 WT (black) and 24 AML (red) mice were weighed. Median weights for each group are displayed on the right side of plot. Significance determined by Mann–Whitney t-test. B Blood isolated from 24 WT (black) and 20 AML (red) mice were stained for live CD11b+ cells and evaluated by flow cytometry. Significance determined by Mann–Whitney t-test. C Representative H&E staining of WT and AML mice-derived spleen (top row, 500 µm scale), liver (middle row, 200 µm scale), and bone marrow (bottom row, 100 µm scale). D Splenocytes from untreated 7 WT (black) and 5 AML (red) mice were assessed for expression (median fluorescence intensity) of markers of immune exhaustion on CD4⁺ T cells (Live, CD11b-, CD3⁺, CD4⁺). Top row, left to right: CTLA-4, TBET, CD44, PD1). Bottom Row, left to right: EOMES, LAG3, TIGIT, TCF1). Significance determined by multiple Mann–Whitney t-tests. CD44, PD1, LAG3 are significantly increased in AML mice. CTLA-4, EOMES, and TCF1 are significantly decreased in AML mice. E Splenocytes from untreated WT (black) and AML (red) mice were assessed for expression (median fluorescence intensity) of markers of immune exhaustion on CD8⁺ T cells, as in (D). Significance determined by multiple Mann–Whitney t-tests. CD44, EOMES, and TIGIT are significantly increased in AML mice.