Fig. 4: Clonogenic capacity of CD34+ cells in JAK2-mutated MF patients. | Leukemia

Fig. 4: Clonogenic capacity of CD34+ cells in JAK2-mutated MF patients.

From: Chromosome 9p trisomy increases stem cells clonogenic potential and fosters T-cell exhaustion in JAK2-mutant myeloproliferative neoplasms

Fig. 4

A Total number of colonies obtained from methylcellulose-based clonogenic assays (n = 7 HET, n = 7 HOM, n = 6 + 9p). B Total number of colonies obtained from collagen-based clonogenic assays (n = 7 HET, n = 7 HOM, n = 6 + 9p). C Percentage of the different Colony-Forming Units (CFUs) obtained from methylcellulose-based clonogenic assays. D Percentage of different Colony-Forming Units obtained from collagen-based clonogenic assays. Results are represented as mean + SEM. Evaluation of POU5F1 (E) and NANOG (F) expression at the transcriptional level in CD34+ cells from JAK2-mutated MPN patients and healthy donors (n = 5 HD, n = 6 HET, n = 7 HOM, n = 8 + 9p). Each barplot represents the relative quantity of the target mRNA quantified by means of qRT-PCR. Abbreviations: RQ = relative quantity; CFU-E/BFU-E = Colony-Forming Unit—Erythroid/Burst-Forming Unit—Erythroid; CFU-GM = Colony-Forming Unit—Granulocyte/Macrophage; CFU-G = Colony-Forming Unit—Granulocyte; CFU-M = Colony-Forming Unit—Macrophage; CFU-GEMM = Colony-Forming Unit—Granulocyte/Erythrocyte/Macrophage/Megakaryocyte; CFU-MK = Colony Forming Unit Megakaryocyte; CFU-NON MK = Colony Forming Unit Non-Megakaryocyte; CFU-MIX = Mixed CFU-Mk/Non CFU-Mk colonies; HD = healthy donors; HET = JAK2-mutated heterozygous patients; HOM = JAK2-mutated homozygous patients; +9p = JAK2-mutated patients with 9p trisomy. *p < 0.05; **p < 0.01.

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