Fig. 7: T-cell exhaustion in MPN patients and healthy donors. | Leukemia

Fig. 7: T-cell exhaustion in MPN patients and healthy donors.

From: Chromosome 9p trisomy increases stem cells clonogenic potential and fosters T-cell exhaustion in JAK2-mutant myeloproliferative neoplasms

Fig. 7

A Representative gating strategy used to quantify CD3 + CD8 + CD57-PD-1+ cells. (i) Side scatter area (SSC-A) vs forward scatter area (FSC-A) plot, showing the lymphocyte gate. (ii) Forward scatter height (FSC-H) vs forward scatter area (FSC-A) plot defining the single cell gate. (iii) LIVE/DEAD vs FSC-A plot gating for live cells. (iv) CD8-FITC vs CD3-APC Vio770 plot depicting CD3 + CD8+ cells. (v) CD57-Alexa Fluor 647 vs PD-1 BV421 plot, showing the percentage of PD-1 + CD57- cells. Flow-cytometric assessment of circulating exhausted CD3 + CD8 + CD57-PD1+ (B), CD3 + CD8 + PD1 + LAG3+ (C), CD3 + CD8 + PD1 + TIM3+ (D), CD3 + CD8 + PD1 + CD244+ (E), CD3 + CD8 + PD1 + CTLA4+ (F) T cells in MPN patients and healthy donors. Results are represented as means ± SEM. HD: healthy donors; HET: JAK2-mutated heterozygous patients; HOM: JAK2-mutated homozygous patients; +9p: JAK2-mutated patients with 9p trisomy.

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