Fig. 4: Selective deletion of CDKL5 from Satb2+ callosal projection neurons display similar callosal deficits.
From: CDKL5 sculpts functional callosal connectivity to promote cognitive flexibility

a Schematic of breeding cross of Satb2cre/+ and CDKL5fl/fl mouse lines. b (Top) Patch clamp recordings schematic of P15 L5 pyramidal neurons in the cingulate cortex following corpus callosum stimulation. (Bottom) Example traces of minimal and maximal eEPSP responses. c Fiber fraction at P15 demonstrating an increase in the number of callosal inputs in conditional KO (cKO) mice (Mann Whitney p = 0.0072**, Ctrl: 0.158 ± 0.019, n = 56 cells/8 mice; cKO: 0.069 ± 0.011, n = 31 cells/6 mice). d (Top) Schematic of VSDI configuration (Bottom) Example VSDI images of cingulate activity following electrical corpus callosum fiber stimulation. e Peak DF/F signal in layer 2/3 across increasing stimulation amplitudes of callosal fibers (Two-Way Anova REML, Interaction of factors: Genotype and Stimulation intensity, p = 0.7519). (Insert) DF/F response over time at high stimulation (0.5 mA) (Two-way Anova, interaction Genotype × Time, p < 0.0001***; Ctrl: n = 11 slices/9 mice, KO: n = 12 slices/9 mice). f Patch clamp schematic recordings of layer 5 cingulate pyramidal neurons from P15 (Top) with example traces of mEPSC (Bottom). g Frequency of mEPSC (Mann Whitney p = 0.2058, Ctrl: 0.151 ± 0.016, n = 59 cells/3 mice; cKO: 0.127 ± 0.013, n = 75 cells/3 mice). h mEPSC amplitude (Mann Whitney p = 0.1608, Ctrl: 10.92 ± 0.104, n = 59 cells/3 mice, cKO: 11.39 ± 0.172, n = 79 cells/3 mice). i (Top) VSDI configuration illustration. (Bottom) Example VSDI images of peak responses following local layer 5/6 fiber stimulation. j Peak DF/F responses in L2/3 following increasing stimulation intensity (Two-Way Anova, REML, interaction genotype × stimulation amplitude, p = 0.9912, Ctrl: n = 12 slices/6 mice, cKO: n = 11 slices/6 mice). (Insert) DF/F response over time at high stimulation (0.25 mA) (Two-Way Anova, interaction genotype × time, p = 0.7429).