Fig. 6: Cognitive impairment rescued in CDKL5 specific expression in Satb2 + CPN.
From: CDKL5 sculpts functional callosal connectivity to promote cognitive flexibility
a Latency to platform during the five trials in the learning phase. CDKL5STOP × Satb2cre/+ mice learn at the same pace as controls, and both of these groups are significantly different to CDKL5STOP × Satb2+/+ deficient mice (two-way Anova, genotype p = 0.0002***, significant trial 2–5#). b Probe test reveals memory impairment in Stop × Satb2+/+ mice (two-way Anova, Sidak’s multiple comparison between opposite vs expected quadrant: Ctrl p < 0.0001***, Stop × Satb2+/+ p = 0.0667, Stop x Satb2cre/+ p = 0.0022**). Ctrl: n = 29 mice (Satb2 cre- and Satb2 cre+ CDKL5 WT controls combined), StopxSatb2+/+: n = 20 mice, Stop × Satb2cre/+: n = 15 mice. c Latency to platform in reversal test (two-way Anova, interaction genotype × trial, p = 0.912). Ctrl: n = 13 mice, StopxSatb2+/+: n = 13 mice, Stop × Satb2cre/+: n = 8 mice. d Four-choice foraging task paradigm. e (Left) Number of trials needed to reach criterion during the discrimination task (Ctrl: 29.9 ± 1.6, StopxSatb2+/+: 27.3 ± 2.0, Stop × Satb2cre/+: 32.5 ± 2.7, One-way Anova, p = 0.2596). (Right) Total number of errors during the discrimination task (Ctrl: 16.0 ± 1.3, StopxSatb2+/+: 15.3 ± 1.7, Stop x Satb2cre/+: 17.40 ± 1.8, One-way Anova, p = 0.6869). f (Left) Number of trials to reach criterion during the reversal phase (Ctrl: 37.5 ± 2.5, StopxSatb2+/+: 42.4 ± 3.4, Stop × Satb2cre/+: 42.8 ± 3.4, One-way Anova, p = 0.3475). (Right) Total number of errors during the reversal task (Ctrl: 24.2 ± 2.4, StopxSatb2+/+: 29.5 ± 3.4, Stop × Satb2cre/+: 29.9 ± 3.3, One-way Anova, p = 0.2715). g (Left) Number of trials to reach criterion in the spatial shift (Ctrl: 65.5 ± 4.9, Stop × Satb2+/+: 85.4 ± 5.7, Stop × Satb2cre/+: 66.6 ± 6.3, One-way Anova, p = 0.0311*). (Right) Total number of errors during the task (Ctrl: 36.2 ± 3.2, StopxSatb2+/+: 51.4 ± 3.7, Stop × Satb2cre/+: 35.1 ± 3.9, One-way Anova, p = 0.0059**). Ctrl: n = 26 mice, StopxSatb2+/+: n = 16mice, Stop × Satb2cre/+: n = 15mice. #See supplementary table for detailed statistics. Mean ± SEM.
