Fig. 2: Cell atlas of adult Drosophila brain and the biological pathways responsive to MPH and ATX. | Molecular Psychiatry

Fig. 2: Cell atlas of adult Drosophila brain and the biological pathways responsive to MPH and ATX.

From: The effects of methylphenidate and atomoxetine on Drosophila brain at single-cell resolution and potential drug repurposing for ADHD treatment

Fig. 2

A UMAP clustering and visualization of scRNASEQ data. Cells were clustered based on their expression pattern using the unsupervised shared nearest neighbor (SNN) clustering algorithm. Individual dots represent a single cell, and the color of the dot represents the cluster to which the cell belongs. Identification of cell types from clusters (shown in the right panel) was performed by canonical marker and top gene annotation from the literature. Clusters were classified based on function. B Venn diagram showing the numbers of total, unique, and shared DEGs (logfc.threshold = 0.25 & Bonferroni-adjusted P \(\le\) 0.05) following MPH and ATX treatment. C Shared pathways of the common DEGs between MPH and ATX treatment. D Statistics for the number of DEGs in each cluster following MPH and ATX treatment. E Pathway analysis of selected neuronal clusters (C14, C18, and C20) by Metascape. Color bar represents the -log(q-value) of the pathway calculated using Metascape.

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