Fig. 5: Subdivision of glial cells in adult Drosophila brain and their response to psychotropic drugs.

A Re-clustering of the cells labeled in the dim plot (Fig. 2A). Only the subglial cells (ensheathing, astrocyte-like, surface, and cortex glia) are shown and analyzed. B Dot plot (40 genes in total) showing the top 10 genes with positive expression in each distinguished glial population (pairwise comparisons for genes expressed in >25% of cells in either cluster; Log2 FC > 0.25, MAST test with Bonferroni-adjusted P  ≤ 0.05). Dot diameter represents the fraction of cells expressing the gene (row value) in the cell cluster (column value), as shown in the scale. Color intensity represents the average normalized expression level. C The proportion of glial cell subtypes following each of the three treatments are displayed. D Statistics of the DEGs in different subglia. DEGs are mainly enriched in astrocyte-like and ensheathing glia.