Fig. 4: Impaired spontaneous electrical activity and synaptic network formation in ABCA7−/− iPSC-derived neurons.
From: ABCA7 deficiency causes neuronal dysregulation by altering mitochondrial lipid metabolism
A Spontaneous firing patterns in neurons derived from isogenic control and ABCA7−/− iPSCs were measured by MED64 PRESTO 7 weeks after differentiation from NPCs. Each spike is indicated with a yellow arrow. B The frequency of spontaneous firing was monitored in the iPSC neurons for 7 weeks after differentiation (n = 9–10 technical replicates from 2 isogenic lines (#1 and #2)/group). C Extracellular recordings of spontaneous firing including burst firing (red rectangles) in the iPSC-derived neurons were measured by MED64 PRESTO 7 weeks after differentiation. D The incidence of burst firing was monitored in the iPSC neurons for 7 weeks after differentiation (n = 9–10 technical replicates from 2 isogenic lines (#1 and #2)/group). Data represents mean ± SEM. *p < 0.05, ***p < 0.001 by two-tailed student’s t-test at week 7.
