Fig. 1: Opposing effects of ELA and 5HT1A autoreceptor knockdown on adult 5HT neuron activity. | Neuropsychopharmacology

Fig. 1: Opposing effects of ELA and 5HT1A autoreceptor knockdown on adult 5HT neuron activity.

From: Sex-specific and developmental effects of early life adversity on stress reactivity are rescued by postnatal knockdown of 5-HT1A autoreceptors

Fig. 1

a Schematic of the genetic knockdown of 5-HT1A autoreceptors in Pet1-tTS- mice (“WT”) vs. Pet1-Tts+ (“5HT1A K.D.”). b Experimental timeline of ELA (P3-10) and tissue collection for RNAscope. c Representative images of RNAscope for 5HT1A and TPH2 in WT and 5HT1A K.D. mice. d Characterization of 5HT1A K.D. in females at P3, 10, 35, and 56. e Characterization of 5HT1A K.D. in males at P3, 10, 35, and 56. f Representative images of RNAscope for cFos and TPH2 in P56 females. Arrows indicate overlap between cFos and TPH2. g At P35, ELA or 5HT1A K.D. do not affect the number of cFos + /TPH2+ cells in females, or h in males. i At P56, ELA reduces the number of cFos + /TPH2+ cells in WT females while 5HT1A K.D. rescues this effect of ELA. Brackets indicate significant Tukey posthoc tests. j 5HT1A K.D. increases the number of cFos + /TPH2+ cells in adult males. Bracket indicates significant main effect of 5HT1A K.D. Mean ± SEM; *P < 0.05, **P < 0.01, ***P < 0.001.

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