Fig. 3: 5-HT1A autoreceptor knockdown rescues PVN hyperactivity and CRF release in ELA-exposed females.

a Experimental timeline. b Schematic and representative image of cFos+ cells in the PVN. c ELA increases the number of cFos+ cells in the PVN of WT females compared to standard-reared control females. This effect was prevented in ELA-exposed 5-HT1A K.D. females. d ELA or 5-HT1A K.D. did not affect cFos+ cells in the male PVN. e ELA increased CRF in the PVN of WT females compared to controls. This effect was prevented in ELA-exposed 5-HT1A K.D. females. f ELA or 5-HT1A K.D. did not affect CRF protein levels in the male PVN. Mean ± SEM; *P < 0.05.