Fig. 5: 5-HT1A autoreceptor knockdown rescues ELA effects on microglia activation in the female PVN and vDG. | Neuropsychopharmacology

Fig. 5: 5-HT1A autoreceptor knockdown rescues ELA effects on microglia activation in the female PVN and vDG.

From: Sex-specific and developmental effects of early life adversity on stress reactivity are rescued by postnatal knockdown of 5-HT1A autoreceptors

Fig. 5

a Experimental timeline. b Representative images of Iba1+ microglia (green) and CD68+ active microglia (red) in the female vDG. Arrowheads indicate CD68+/Iba1+ active microglia. c–f Activated microglia in the PVN c. ELA or 5HT1A K.D. did not affect the % of active microglia in adolescent females or d males. e ELA increased the % active microglia in the PVN of adult WT females compared to controls. This effect of ELA was prevented in 5HT1A K.D. females. f ELA or 5HT1A K.D. did not affect the % active microglia in the PVN of adult males. Activated microglia in the vDG. g 5HT1A K.D. decreased the % active microglia in the vDG of adolescent females regardless of ELA exposure. h ELA or 5HT1A K.D. did not affect the % of active microglia in adolescent males. i ELA increased the % active microglia in the vDG of adult WT females compared to controls. This effect of ELA was prevented in 5HT1A K.D. females. j ELA or 5HT1A K.D. did not affect the % active microglia in adult males. Mean ± SEM; *P < 0.05; **P < 0.01.

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